qRT-PCR has widely been used for over ten years and has entered into domains with medical needs. The currently used methods require the use of experts to evaluate the result of noise removal, because the methods need some parameters which correlate to the detected noise. The adaption, calibration, can only be executed by a manual process. This fact prevents the usage of the qRT-PCR-analysis in an automated industrial environment. The focus of this article lies in the development of automated pre-processing method which can reduce the significantly influence of the noise. The project ProDIAP (support code 01RI0709A-C) used 640 references for the evaluation. The evaluation of the quality of the qualitative analysis with the Bézier smoothing estimated with the criteria: recall, precision and the specificity. The results of the recall is 100%, the average of the precision is 96% and the average of the specificity is 97%.
